This study demonstrated the utility of Single Standard Conformation Polymorphism (SSCP) markers for assessing the genetic relatedness of 107 wild and cultivated Hevea brasiliensis accessions. Seventeen primer pairs were designed from 15 cDNA nucleotide sequences of H. brasiliensis from the GenBank database. Of these, two primers did not amplify PCR products whereas another two primers generated complicated banding patterns. The remaining 13 primers produced different PCR product fragment lenghts ranging from 200 to 1600 bp. A total of 113 bands and 96 polymorphic bands were detected over all accessions. Three primers (HBGGPPS1), HBGGPPS2, and HGN1) demonstrated monomorphic bands in the cultivated clones. A genetic similarity dendrogram was constructed, using UPGMA, and a high genetic similarity coefficient was detected ranging from 0.75 to 1.00 (average of 0.8343). The 107 Hevea accessions could be divided into 5 clusters based on their genetic similarity coefficients. Results of SSCO marker analyses clearly showed that cultivated clones gave the lowest amount of polymorphism and were closely related to Mato Grosso accessions. Most of the wild accessions were discriminated and the results agree with the previous diversity study based on geographical origins. SSCP markers provided a simple, inexpensive and sensitive method for detecting nucleotide sequence variation in expressed genes and for screening parental materials to extend the H. brasiliensis genetic base in breeding programs.