Evaluation of genetic relatedness of wild and cultivated Hevea brasiliensis accessions with SSCP markers (Record no. 58932)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02199nam a2200181Ia 4500 |
| 100 ## - MAIN ENTRY--AUTHOR NAME | |
| Personal name | Lekawipat Napawan |
| 245 #0 - TITLE STATEMENT | |
| Title | Evaluation of genetic relatedness of wild and cultivated Hevea brasiliensis accessions with SSCP markers |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Name of publisher | Proceedings of IRRDB Conference: NR Industry: Responding to Globalization, 7-8 September 2004, Kunming International Convention & Exhibition Center, China. pp.89-97. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc | This study demonstrated the utility of Single Standard Conformation Polymorphism (SSCP) markers for assessing the genetic relatedness of 107 wild and cultivated Hevea brasiliensis accessions. Seventeen primer pairs were designed from 15 cDNA nucleotide sequences of H. brasiliensis from the GenBank database. Of these, two primers did not amplify PCR products whereas another two primers generated complicated banding patterns. The remaining 13 primers produced different PCR product fragment lenghts ranging from 200 to 1600 bp. A total of 113 bands and 96 polymorphic bands were detected over all accessions. Three primers (HBGGPPS1), HBGGPPS2, and HGN1) demonstrated monomorphic bands in the cultivated clones. A genetic similarity dendrogram was constructed, using UPGMA, and a high genetic similarity coefficient was detected ranging from 0.75 to 1.00 (average of 0.8343). The 107 Hevea accessions could be divided into 5 clusters based on their genetic similarity coefficients. Results of SSCO marker analyses clearly showed that cultivated clones gave the lowest amount of polymorphism and were closely related to Mato Grosso accessions. Most of the wild accessions were discriminated and the results agree with the previous diversity study based on geographical origins. SSCP markers provided a simple, inexpensive and sensitive method for detecting nucleotide sequence variation in expressed genes and for screening parental materials to extend the H. brasiliensis genetic base in breeding programs. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Hevea brasiliensis |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | SSCP markers |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Teerawatanasuk Kanikar |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Toojinda Teerayuth |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Tragoonrung Somvong |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Vanavichit Apichart |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Journals |
| Withdrawn status | Lost status | Damaged status | Not for loan | Home library | Current library | Shelving location | Date acquired | Koha item type |
|---|---|---|---|---|---|---|---|---|
| Journals | RRII Library | RRII Library | Biotechnology | 17/12/2021 | Journals |