Source Year: 2013

Natural rubber latex sera have been investigated for anti-proliferative property on cancer cell lines. The present report describes fractionaation of latex B- and C-serum using cell viability assay(anti-proliferative activity) as a guide to narrow down on the active constituents. Dialysis of B-and C-serum against distilled water caused some of its constituents to precipitate, then a brief centrifugation separated their fractions- dialysed B-serum percipitate (DCP) nad dialyesd C-serum supernanatant (DCS). Boiling of B- and C-serum resulted in extensive precipitation; a brief centrifugation separated the boiled B-and C-serum precipitate from their supernantant. Cell viability assy performed on two human cancer-origin cell lines revealed a marked increase in anti-proliferative activity in the dialysed C-serum fractions and in DBP but diminished in DBS as well as in the boiled B-and C-serum fractions. Chromatographic separation of DBP, DCP and DCS followed by cell viability assy will shed light if sub-fractions with further improved anti-proliferative activity are attainable.