Cell viability assay guided fractionation of natural rubber latex sera (Record no. 55251)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 01708nam a2200229Ia 4500 |
| 100 ## - MAIN ENTRY--AUTHOR NAME | |
| Personal name | Sunderasan E |
| 245 #0 - TITLE STATEMENT | |
| Title | Cell viability assay guided fractionation of natural rubber latex sera |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Name of publisher | Journal of Rubber Research |
| Year of publication | 2013 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Number of Pages | 195-202 |
| 500 ## - GENERAL NOTE | |
| General note | Source Year: 2013 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc | Natural rubber latex sera have been investigated for anti-proliferative property on cancer cell lines. The present report describes fractionaation of latex B- and C-serum using cell viability assay(anti-proliferative activity) as a guide to narrow down on the active constituents. Dialysis of B-and C-serum against distilled water caused some of its constituents to precipitate, then a brief centrifugation separated their fractions- dialysed B-serum percipitate (DCP) nad dialyesd C-serum supernanatant (DCS). Boiling of B- and C-serum resulted in extensive precipitation; a brief centrifugation separated the boiled B-and C-serum precipitate from their supernantant. Cell viability assy performed on two human cancer-origin cell lines revealed a marked increase in anti-proliferative activity in the dialysed C-serum fractions and in DBP but diminished in DBS as well as in the boiled B-and C-serum fractions. Chromatographic separation of DBP, DCP and DCS followed by cell viability assy will shed light if sub-fractions with further improved anti-proliferative activity are attainable. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | anti-proliferative activity |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Cell viability assay |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Latex B-serum |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Latex C-serum |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Lam K L |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ong M T |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Rahman Norazreen Abd |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Yang K L |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Journals |
| Withdrawn status | Lost status | Damaged status | Not for loan | Home library | Current library | Date acquired | Serial Enumeration / chronology | Koha item type |
|---|---|---|---|---|---|---|---|---|
| Journals | RRII Library | RRII Library | 21/03/2014 | Volume 16, Issue 3 | Journals |