Identifying, cloning and sequencing of a RAPD marker linked to mildew-resistance gene of Hevea brasiliensis (Record no. 60683)

MARC details
000 -LEADER
fixed length control field 01252nam a2200229Ia 4500
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Personal name Shoucai Chen
245 #0 - TITLE STATEMENT
Title Identifying, cloning and sequencing of a RAPD marker linked to mildew-resistance gene of Hevea brasiliensis
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Name of publisher Paper presented at IRRDB, China, 1999
520 ## - SUMMARY, ETC.
Summary, etc The genomic DNA isolated from 11 mildew (Oidium hevea)-resistant and 11 susceptible clones of Hevea brasiliensis were analyzed with 52 random primers by using RAPD technique. A DNA fragment designated OPV-10 390 amplified from the genomic DNA from RRTC52 (CK) demonstrated that this sequence was low copy sequence in the resistant clones but not contained in the susceptible clones. It can thus be seen that OPV-10 390 can be used as RAPD markers linked to mildew-resistance gene. OPV-10 390 was cloned and sequenced, and analyzed by Blastn. Results showed that this fragment was a new sequence.
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Topical Term Cloning
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Topical Term Hevea brasiliesis
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Topical Term Identification
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Topical Term Mildew-resistance gene
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Topical Term RAPD marker
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Topical Term Sequence analysis
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Dogqiong Hu
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Personal name Hanshuang Shao
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Personal name Shaoping Fu
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Personal name Xueqin Zheng
942 ## - ADDED ENTRY ELEMENTS (KOHA)
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