An Efficient method for the isolation of good quality RNA from bark tissues of mature rubber trees (Record no. 54121)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 01389nam a2200241Ia 4500 |
| 100 ## - MAIN ENTRY--AUTHOR NAME | |
| Personal name | Sathik M B M |
| 245 #3 - TITLE STATEMENT | |
| Title | An Efficient method for the isolation of good quality RNA from bark tissues of mature rubber trees |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Name of publisher | Journal of Rubber Research |
| Year of publication | 2005 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Number of Pages | 182-189 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc | In this study, an attempt has been made to extract good quality RNA from soft bark tissues of Hevea which generally contain plenty of interfering compounds such as phenolic compounds, tannins, lignins and carbohydrates. The method explained here involes removal of phenolic compounds using polyvinyl pyrrolidone and selective precipitation of RNA using lithium chloride. The purity of RNA prepared by this method was tested for its ability to get a good quality cDNA prepared. The cDNA produced from total RNA preparation was further con firmed by amplification of specific house keeping genes using quantitative real time PCR method. This protocol appears best suited for Hevea bark that is rich in several contaminating factors. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | cDNA |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Real time PCR |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Recalcitrant bark tissue |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | RRII 105 |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Tapping panel dryness |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Total RNA isolation |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Jacob J |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Nataraja K N |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Thomas M |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Udayakumar M |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Journals |
| Withdrawn status | Lost status | Damaged status | Not for loan | Home library | Current library | Shelving location | Date acquired | Serial Enumeration / chronology | Koha item type |
|---|---|---|---|---|---|---|---|---|---|
| Journals | RRII Library | RRII Library | Plant physiology | 17/12/2021 | Volume 8, Issue 3 | Journals |