000			01967nam a2200265Ia 4500
008			220216s9999 xx 000 0 und d
100			_aLardet L
245		0	_aVariation in GUS activity in vegetatively propagated Hevea brasiliensis transgenic plants
260			_bPlant Cell Reports _c2011
300			_a1847-1856
520			_aHevea brasiliensis transgenic plants are regenerated from transgenic callus lines by somatic embryogenesis. Somatic embryogenesis is not yet available for commercial propagation of Hevea clones, which requires conventional grafting of buds on rootstock seedlings (buddings). The stability of transgene expression in budded plants is therefore necessary for further development of genetic engineering in rubber trees. Transgene expression was assessed by fluorimetric beta-glucuronidase (GUS) activity in fully developed leaves of in vitro plants from transgenic lines and their sub-lines obtained by budding. A large variation in GUS activity was found in self-rooted in vitro plants of five transgenic lines, and the absence of activity in one line suggested transgene silencing. Beyond confirming transmissibility of the reporter gene by budding and long-term expression, a quantification of GUS acitivty revealed that greater variability existed in budded plants compared to self-rooted mother in vitro plants for three transgenic lines. Although somatic embryogenesis provided more stable GUS activity, budding remained an efficient way of propagating transgenic plants but transgene expression in budded plants should be verified for functional analysis and further development.
650			_aBeta-glucuronidase
650			_aGene silencing
650			_aGenetic transformation
650			_aGrafting
650			_aRubber tree
650			_aTransgene
700			_aBenistan E
700			_aDessailly F
700			_aLeclercq J
700			_aMartin F
700			_aMontoro P
700			_aOliver G
942			_cJS
999			_c72101 _d72101