| 000 | 02273nam a2200265Ia 4500 | ||
|---|---|---|---|
| 008 | 220216s9999 xx 000 0 und d | ||
| 100 | _aSingh A P | ||
| 245 | 4 | _aThe Micromorphology and protein characterization of rubber particles in ficus carica, ficus benghalensis and Hevea brasiliensis | |
| 260 |
_bJournal of Experimental Botany _c2003 |
||
| 300 | _a985-992 | ||
| 520 | _aRubber biosynthesis takes place on the surface of rubber particles. These particles are surrounded by a monolayer membrane in which the rubber transferase is anchored. In order to gain better insight into whether rubber particles from different plant species share common structural characteristics, the micromorphology of rubber particles from Ficus carica, Ficus benghalensis, and Hevea brasiliensis was examined by electron microscopy. Rubber particles of all three species wew spherical in shape, and the size of rubber particles of H. brasiliensis was much smaller than those of F. carica and F. benghalensis. In addition, investigations were undertaken to compare the cross-reactivity of the antibody raised against either the H. brasiliensis small rubber particle protein (SRPP) which is suggested to be nvolved in rubber biosynthesis, or the cis-prenyltransferase (CPT) which has an activity similar to rubber tarnsferase. Both western analysis and TEM-immunogold labelling studies showed that rubber particles of F. carica and F. benghalensis do not contain the SRPP. None of the rubber particles in F. carica, F. benghalensis and H. brasiliensi contained the CPT, suggesting that the CPT itself could not catalyse the formation of high molecular weight rubber. These results indicate that rubber particles in the three different plant species investigated share some degree of similarity in architecture, and that the SRPP and CPT themselves are not the core proteins necessary for rubber biosynthesis. | ||
| 650 | _aElectron microscopy | ||
| 650 | _aFicus benghalensis | ||
| 650 | _aFicus carica | ||
| 650 | _aHevea brasiliensis | ||
| 650 | _aImmunolocalization | ||
| 650 | _aMicromorphology | ||
| 650 | _aRubber particle | ||
| 650 | _aRubber particle protein | ||
| 700 | _aChung G C | ||
| 700 | _aKang H | ||
| 700 | _aKim Y S | ||
| 700 | _aWi S G | ||
| 942 | _cJS | ||
| 999 |
_c70858 _d70858 |
||