000			01475nam a2200217Ia 4500
008			220216s9999 xx 000 0 und d
100			_aBonfils F
245		0	_aSteric exclusion chromatography study of natural rubber films prepared from fresh field latex
260			_bJournal of Natural Rubber Research _c1995
300			_a143-153
520			_aCyclohexane was used to replace tetrahydrofuran (THF) as the elution phase in analysis of natural rubber by steric exclusion chromatography. The films, which were prepared from fresh field latex and dissolved in cyclohexane, had high gel rates up to 50;in some cases and overestimated average molecular weights. These problems were linked to certain hydrophilic non-rubber constituents and more particularly to the crosslinking they seem to bring about under certain conditions. This study showed that these crosslinking, which are not covalent, could be overcome by adding hydroxylamine neutral sulphate to the latex prior to film preparation, or by washing the films in deionised water before dissolving them. Using a mixed solvent, such as a cyclohexane and THF mixture (95/5,;v/v), to dissolve this type of samples also significantly limited the proportion of gel.
650			_aHevea brasiliensis
650			_aLatex
650			_aSteric exclusion chromatography
700			_aAllet Don A
700			_aKoman Achi A
700			_aLaigneau J C
700			_aSainte Beuve J
700			_aSylla X
942			_cJS
999			_c69315 _d69315