000			02234nam a2200181Ia 4500
008			220216s9999 xx 000 0 und d
100			_aHuang T D _cet al
245		0	_aSomatic embryo, an alternative target tissue for Agrobacterium-mediated transformation in Hevea brasiliensis
260			_bJournal of Rubber Research _c2015
300			_a171-188
500			_aSource Year: 2015
520			_aTraditionally, embryogenic callus has been used as the only receptor source of genetic transformation in Hevea brasiliensis. In this study, it showed that somatic embryos were a viable alternative to embryogenic callus. The appropriate concentration of both decontamination and selection antibiotics was firstly screened. Visual and cytological observations found both epidermal and subepidermal cotyledon cells could be infected, indicating that somatic embryos were susceptible to antibiotics and Agrobacterium. Subsequently, five isolated consecutive transformation experiments were carried out to produce the transgenic plants using a total of 1158 somatic embryos of which 47 (4.06;) GUS-positive embryos were produced after cell differentiation and embryogenesis. Six were successfully multiplied and produced 33 plants. PCR and Southern blot analysis confirmed that the T-DNA was integrated into the recovered plants. Finally, three additional cycles of secondary embryogenesis were carried out for transgenic embryo proliferation efficiency assessment. The rate of proliferation tended to slightly increase with each proliferation generation, and one initial transgenic embryo-derived GUS-negative embryo were observed in the fifth multiplication cycle, indicating that transgenic plants could be propagated through secondary embryogenesis adn some initial transgenic embryos might be chimeric. This is the first attempt on producing transgenic plants using somatic embryos as the target tissue in H. brasiliensis. It is demonstrated that somatic embryos can be used as an alternative target tissue for Agrobacterium-mediated transformation in Hevea brasiliensis.
650			_aAgrobacterium tumefaciens
650			_aGenetic transformation
650			_aSecondary embryogenesis
650			_aSomatic embryo
942			_cJS
999			_c68902 _d68902