| 000 | 00962nam a2200181Ia 4500 | ||
|---|---|---|---|
| 008 | 220216s9999 xx 000 0 und d | ||
| 100 | _aWajant H | ||
| 245 | 0 | _aPurification and characterization of hydroxynitrile lyase from Hevea brasiliensis | |
| 260 |
_bPlant science _c1996 |
||
| 300 | _a25-31 | ||
| 520 | _aHydroxynitrile lyase from Hevea brasiliensis (HbHNL) was purified to apparent homogeneity. Purified HbHNL consists of non-covalently linked monomers of 30kDa. HbHNL exhibits a typical Michaelis-Menten kinetics with a Km of 115 mM and the enzyme activity is strongly inhibited by diisopropyl fluorophosphate and diethyl pyrocarbonate indicating a serine and histidine in the active site. HbHNL is serologically related to the HNL from Manohot esculenta but not to other HNLs. | ||
| 650 | _aCyanogenesis | ||
| 650 | _aEnzyme purification | ||
| 650 | _aHevea brasiliensis | ||
| 650 | _aHydroxynitrile lyase | ||
| 700 | _aForster S | ||
| 942 | _cJS | ||
| 999 |
_c66108 _d66108 |
||