000			01740nam a2200145Ia 4500
008			220216s9999 xx 000 0 und d
100			_aTian Wei-Min
245		0	_aProtein-storing cells with 67 kDa protein in regularly tapped Hevea trees and in trees affected by tapping panel dryness
260			_bJournal of Rubber Research _c1999
300			_a183-191
520			_aProtein-storing cells (PSCs) are found in secondary phloem of Hevea trees. In this study it was found that a 67 kDa protein based on SDS-PAGE might be the main component of the vacuole proteins in PSCs. SDS-PAGE analysis indicated that the 67 kDa protein varied in abundance at different levels of tree trunk in untapped and tapped trees, and that the protein also changed when tapped trees were affected by tapping panel disease (TPD). These changes were consistent with that of the amount of PSC vacuole proteins detectable by microscopy. The indirect immunocytochemistry in light microscopical level gave a more reliable evidence that the 67 kDa protein is quantitatively the more important component of the PSC vacuole inclusion. Latex exploitation caused a marked decrease in relative abundance of 67 kDa protein in the area under the tapping cut of the tree trunk. In comparison with healthy tapped trees, the trees which were affected by TPD and rested from tapping had much more 67 kDa protein in the bark tissues near the tapping cut. These facts indicate that the 67 kDa protein may be utilised as storage material for latex regeneration in the tapped trees and that the relatively abundant 67 kDa protein in TPD trees may be caused by resting the trees from latex exploitation.
650			_aTapping panel dryness
700			_aHao Bing-Zhong
942			_cJS
999			_c66068 _d66068