000			01386nam a2200157Ia 4500
008			220216s9999 xx 000 0 und d
100			_aCheong Kay Fong
245		0	_aNucleotide sequence of the chloroplast gene for the large sub-unit of ribulose-1,5-bisphosphate carboxylase/oxygenase from Hevea brasiliensis
260			_bJournal of Natural Rubber Research _c1993
300			_a146-153
520			_aA shortgun genomic library of the chloroplast DNA (ctDNA) of Hevea brasiliensis (clone RRIM 600) was constructed with the plasmid cloning vector pUC19 in Escherichia coli. The gene, rbcL, for the large sub-unit (LS) of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) was isolated by first screening the library by colony hybridisation and then confirmation by Southern hybridization with a radiolabelled DNA probe that contains the internal sequence of rbcL from tobacco, Nicotiana tabacum. Sequence data indicated that the Hevea rbcL gene contains an open reading frame of 1425 nucleotides encoding a protein of 475 amino acid residues. Conserved sequences in the gene and LS protein were detected. Palindromic sequences which may form intrastrand stem-loop structures for the termination of transcription were encountered at the 3 non-coding region of the gene.
650			_aChloroplast gene
650			_aHevea brasiliensis
700			_aKoh Chong Lek
942			_cJS
999			_c64217 _d64217