000			02879nam a2200613Ia 4500
008			220216s9999 xx 000 0 und d
100			_aCastonguay Yves
245		0	_aMolecular physiology and breeding at the crossroads of cold hardiness improvement
260			_bPhysiologia Plantarum _c2013
300			_a64-74
520			_aAlfalfa (Medicago sativa L) is a major forage legume grown extensively worldwide with important agronomic and environmental attributes. Insufficient cold hardiness is a major impediment to its reliable production in northern climates. Improvement of freezing tolerance using conventional breeding approaches is slowed by the quantitative nature of inheritance and strong interactions with the environment. The development of gene-based markers would facilitate the identification of genotypes with superior stress tolerance. Successive cycles of recurrent selection were applied using an indoor screening method to develop populations with significantly higher tolerance to freezing (TF). Bulk segregant analysis of heterogeneous TF populations identified DNA variations that are progressively enriched in frequency in response to selection. Polymorphisms resulting from intragenic variations within a dehydrin gene were identified and could potentially lead to the development of robust selection tools. Our results illustrate the benefits of feedback interactions between germplasm development programs and molecular physiology for a deeper understanding of the molecular and genetic bases of cold hardiness.
650			_aAFLP
650			_aAmplified fragment length polymorphism
650			_aBSA
650			_aBulk segregant analysis
650			_aCBF
650			_aCNV
650			_acold-regulated
650			_aCopy number variation
650			_aCOR
650			_ac-repeat binding factor
650			_ac-repeat/dehydration responsive element
650			_aCRT/DRE
650			_aGBS
650			_agenotyping-by-sequencing
650			_aInDel
650			_ainsertion and deletion
650			_aLD
650			_alinkage disequilibrium
650			_amarker-assisted selection
650			_aMAS
650			_anext-generation sequencing
650			_aNGS
650			_aQTL
650			_aquantitative trait loci
650			_arandom amplified polymorphic DNA
650			_aRAPD
650			_arestriction fragment length polymorphism
650			_aRFLP
650			_aSCAR
650			_asequence related amplified polymorphism
650			_asequence-characterized amplified region
650			_asingle-nucleotide polymorphism
650			_aSNP
650			_aSRAP
650			_aTF
650			_atolerance to freezing
700			_aBertrand Annick
700			_aCloutier Jean
700			_aDube Marie-Pier
700			_aLaberge Serge
700			_aMichaud Real
942			_cJS
999			_c63214 _d63214