000			02025nam a2200193Ia 4500
008			220216s9999 xx 000 0 und d
100			_aMendanha Asseara Batista Lettao
245		0	_aMicropropagation of rubber trees (Hevea brasiliensis Muell. Arg.)
260			_bGenetics and Molecular Biology _c1998
300			_a395-398
500			_aSource Year: 1999
520			_aTissue cultures were established from newly expanded leaves and axillary buds of rubber trees (Hevea brasiliensis Muell. Arg.) . Calli formed from these explants, but no regeneration occurred. Shoots were obtained from axillary buds cultured on Murashige and Skoogs (MS) medium supplemented with 1.0 mg/l kinetin, 1.0 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D), 20 g/l sucrose and 4 g/l Difco agar. Formation of a root similar to a tap root was induced on MS medium supplemented with 5.0 mg/l naphthaleneacetic acid (NAA), 3.0 mg/l inodlylbutyric acid (IBA), 50 g/l sucrose and 4 g/l Difco agar. Several types of explants were used in attempts to recover complete rubber tree plants with well-developed tap roots. Leaf explants and axillary buds formed calli on MS basic medium with different combinations of kinetin, benzylaminopurine (BAP), 2,4-D, IBA, NAA and indolylacetic acid (IAA). The antibiotic tetracycline was also used to control possible bacterial infections. However, no antibiotic effect was noted. Calli formation was abundant, but no regeneration was observed when the calli from different media was transferred to MS medium without growth hormones. On this basic medium, callus cultures became necrotic and died. Shoots developed from axillary buds, rooted vigorously when cultured on MS medium with NAA, IAA, and IBA. Based on these results, further studies with commercially important clones should lead to a feasible micropropagation technique.
650			_aMicropropagation
650			_aRubber tree
700			_aFreire _BAdelson de Barros
700			_aTorres Roberto Augusto de Almeida
856			_uPGRA
942			_cJS
999			_c62928 _d62928