000			01424nam a2200181Ia 4500
008			220216s9999 xx 000 0 und d
100			_aSunderasan E
245		0	_aLatex B-Serum B-1,3-Glucanase (Hev b II) and a component of the microhelix (Hev b IV) are major latex allergens
260			_bJournal of Natural Rubber Research _c1995
300			_a82-99
520			_aTwo allergenic (IgE-binding) proteins have been isolated from the B-serum of natural rubber latex by dialysis-induced protein precipitation followed by gel filtration. One of the allergens, Hev b II, that is specific to the monoclonal antibody USM/RB4, commonly appears as a doublet of molecular weights 34 and 36 kDa under reducing conditions of SDS-polycacrylamide gel electrophoresis. In the absence of 2-mercaptoethanol, a 70 kDa band can also be seen in addition to the doublet. The allergen has a pI of circa 9.5 and is identified as B-1, 3-glucanase. The second allergen, Hev b IV, has a pI at of crica 4.5 and appears under under reducing conditions as a band of 50-57 kDa. In its unreduced form, a principal band of approximately 100 kDa is discernible. Immuno-gold labelling with the monoclonal antibody USM/RB3 shows that Hev B IV is a component of the microhelix protein complex of B-serum.
700			_aCardosa M J
700			_aHamid Sharifah
700			_aHamzah Samsidar
700			_aWard M A
700			_aYeang H Y
942			_cJS
999			_c61993 _d61993