000			01626nam a2200157Ia 4500
008			220216s9999 xx 000 0 und d
100			_aJayashree R
245		0	_aIntegration and expression of hmgr 1 gene in the transgenic plants of Hevea brasiliensis (clone RRII 105)
260			_bPLACROSYM XXI. International Symposium on Plantation Crops, 10-12 December 2014, Kozhikode, India, pp. 52. Abstracts.
520			_aIn Hevea brasiliensis, natural rubber (cis-1, 4-polyisoprene) is produced in the milky cytoplasm of specialized cells called laticifers. The enzyme 3-hydroxy 3-methylglutaryl Coenzyme A reductase (HMGR) catalyzes the first step in the isoprenoid pathway and is considered as a rate limiting enzyme in rubber biosynthesis. Agrobacterium mediated genetic transformation of the embryogenic tissues of zygotic origin using the plasmid vector pBIBhmgr 1 oroduced transgenic plants. The integration of the transgene was confirmed in the plants using southern hybridization. The expression of the transgene was studied in the plants using northern analysis. Higher transcript level was observed in the tested plant compared to the control. The HMGR protein was semi-quantified in the selected transgenic plants using ELISA, where a 2-8 fold increase in the HMGR enzyme activity was noticed. After correlating the HMGR activity with the latex yield in Hevea brasiliensis, this protein parameter can be used as a molecular tool for the early selection of plants in Hevea breeding programmes.
650			_aAgrobacterium
650			_ahmgr1 gene
650			_aRRII 105
650			_aTransgenic plants
942			_cJS
999			_c61482 _d61482