000			01771nam a2200217Ia 4500
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100			_aSushamakumari S
245		0	_aInfluence of growth regulators and sucrose on somatic embryogenesis and plant regeneration from immature inflorescence of Hevea brasiliensis
260			_bIndian Journal of Natural Rubber Research _c2000
300			_a19-29
520			_aA method for somatic embryogenesis and plant regeneration from immature inflorescence of Hevea brasiliensis, clone RRII 105 is described. The influence of different growth regulators and sucrose at various stages of this pathway has also been investigated. Among the different growth regulators tested for callus induction, synergistic effect of the two auxins 2,4-D and NAA was observed. Optimum growth regulator combination for the callus induction was found to be 2,4-D/NAA/KIN (45/27/23 uM). Embryo induction was favoured by both BA and zeatin in the presence of low levels of NAA or 2,4-D. Significant increase in embryo induction frequency was observed in the presence of GA. A higher sucrose level was found to be essential for effective embryo induction as well as maturation. ABA did not show any positive effect on embryo maturation, eventhough lower levels of ABA in the maturation medium enhanced proliferation and further embryogenesis. Plant regeneration frequency was found to be higher on media fortified with BA and GA. Lower sucrose level was found to be beneficial for plant regeneration.
650			_aGrowth regulators
650			_aHevea brasiliensis
650			_aPlant regeneration
650			_aSomatic embryogenesis
700			_aAsokan M P
700			_aJayasree R
700			_aRekha K
700			_aSobha S
942			_cJS
999			_c61238 _d61238