000 00975nam a2200157Ia 4500
008 220216s9999 xx 000 0 und d
100 _aShamsul Bahri A R
245 0 _aImmunocytochemical localisation of rubber membrane protein in Hevea latex
260 _bJournal of Natural Rubber Research
_c1996
300 _a88-95
520 _aImmunogold double labelling technique by electron microscopy was employed to localise the 14 kDa and 24 kDa protein obtained from rubber particle membrane in Hevea latex. These proteins were used to elicit antibodies in rabbits. The two antibodies showed selective labelling on the rubber particles: 14 kDa antibody bound more to the larger rubber particles (>_0.3 mm) whereas the 24 kDa antibody more to the smallerr rubber particles (<_ 0.2 mm). The use of osmium tetroxide to fix rubber particles did not distroy the antigenic sites.
650 _aHevea latex
650 _aRubber membrane
700 _aHamzah Samsidar
942 _cJS
999 _c60700
_d60700