000			01252nam a2200229Ia 4500
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100			_aShoucai Chen
245		0	_aIdentifying, cloning and sequencing of a RAPD marker linked to mildew-resistance gene of Hevea brasiliensis
260			_bPaper presented at IRRDB, China, 1999
520			_aThe genomic DNA isolated from 11 mildew (Oidium hevea)-resistant and 11 susceptible clones of Hevea brasiliensis were analyzed with 52 random primers by using RAPD technique. A DNA fragment designated OPV-10 390 amplified from the genomic DNA from RRTC52 (CK) demonstrated that this sequence was low copy sequence in the resistant clones but not contained in the susceptible clones. It can thus be seen that OPV-10 390 can be used as RAPD markers linked to mildew-resistance gene. OPV-10 390 was cloned and sequenced, and analyzed by Blastn. Results showed that this fragment was a new sequence.
650			_aCloning
650			_aHevea brasiliesis
650			_aIdentification
650			_aMildew-resistance gene
650			_aRAPD marker
650			_aSequence analysis
700			_aDogqiong Hu
700			_aHanshuang Shao
700			_aShaoping Fu
700			_aXueqin Zheng
942			_cJS
999			_c60683 _d60683