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100			_aChung H H _cet al
245		0	_aIdentification and characterisation of endo-1,4-B-glucanase family members in Hevea brasiliensis
260			_bJournal of Rubber Research _c2015
300			_a148-158
500			_aSource Year: 2015
520			_aIn order to study wood formation in Hevea brasiliensis, endo-1,4-B-glucanase (EGase) was selected for molecular genetic analysis. EGase sequences were isolated from bark, leaf and latex transcriptomes (clone RRIM 928). Altogether, 22 sequences were obtained from the bark transcriptome while 14 and 8 sequences were obtained from leaf and latex transcriptomes, respectively. Based on multiple sequence alignment, only twelve sequences encoded full protein open reading frames while the others contained partial proteins. Translation of twelve EGase sequences (designated HbEg1-12) produced peptide sequences ranging from 494-622 amino acids. Based on protein motif conservation and phylogenetic analysis, these EGases were separated into three classes, A, B and C. Expression of EGase was found to be generally low in latex, bark and leaves.
650			_aEndo-1,4-B-glucanase
650			_aRubber wood
650			_aTranscriptome
650			_aWood formation
942			_cJS
999			_c60587 _d60587