000			01659nam a2200217Ia 4500
008			220216s9999 xx 000 0 und d
100			_aSunderasan E
245		0	_aGenetic tansformation of Hevea brasiliensis with hman arial ntriuretic Factor
260			_bJournal of Rubber Research _c2012
300			_a255-264
520			_aAgrobacterium mediated genetic transformation was performed on anther callus of Hevea cultivar GLI with a gene encoding human atrial natriuretic factor (HANF), a peptide hormone that is involved in regulating cardiac blood pressure. The gene constructs containing four different lengths of the indigenous hevein promoter, also incorporated the marker nptII for selection of transformants. Thus far 28 plants were regenerated and their bud-grafted generations were successfully transferred to the soil; all transcript in leaf samples was detected by RT-PCR in a number of original transformants and their vegetative generations, and the authenticity of the amplicon was confirmed by nucleotide sequencing. Polyclonal anti-atrial natriuretic peptide detected a band in the low molecular weigh region in western- immunoblot of leave protein of an original transformed plant, while MALDITOF-TOF and database searches of the corresponding protein band on SDS-gel revealed matches to atrial natriuretic peptide in the C-terminus of HANF.
650			_agenetic transformation
650			_aHevea brasiliensis
650			_ahuman atrial natriuretic factor
650			_aprotein mass-spectra analysis
650			_aRT-PCR
700			_aArokiaraj P
700			_aAzharuddin Azlina
700			_aBadaruddin EBadrul
942			_cJS
999			_c59861 _d59861