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100			_aAttanayaka D P S T G
245		0	_aGenetic polymorphism and expression of the rubber elongation factor gene (ref) in Hevea brasiliensis cultivars
260			_bJournal of the Rubber Research Institute of Sri Lanka _c2013
300			_a62-71
500			_aSource Year: 2013
520			_aRubber Elongation Factor (REF) protein is one of the most abundant proteins found in Hevea latex. Investigation on the organization and expression of the gene encoding REF protein in commercially planted Hevea brasiliensis cultivars is important to shed light on its possible role in rubber biosynthesis, the studies of Hevea latex allergy and in molecular breeding of new rubber clones. RFLP analysis of the Hevea brasiliensis cultivars used in this study, showed the occurrence of the polymorphic forms of the ref gene and its possibility to be used as an important genetic marker in Hevea breeding and selection. The RRIC 100 series clones produced more bands indicating the possibility of occurrence of more ref gene copies. The RFLP analysis of the progeny derived by crossing RRIC 110 and IAN 45/710 proved the nuclear inheritance pattern of the ref gene with two alleles at ref loci E-1 and E-3. The gene expression studies showed the differential expression of the ref gene between leaf and laticiferous tissues with high abundance of ref gene transcripts in lattices of Hevea brasiliensis. The ref gene expression was approximately 100 fold higher in latex than in leaves. The intensities of the hybridisation signals obtained from latex RNA of different clones showed variation implying the existance of the genotypic differences for ref gene expression.
650			_aExpression of ref gene
650			_aFactor
650			_aHevea latex
650			_aRFLPs
650			_aRNA dot-blots
650			_aRubber elongation
942			_cJS
999			_c59848 _d59848