000			01808nam a2200229Ia 4500
008			220216s9999 xx 000 0 und d
100			_aHan Kyung-Hwan
245		0	_aGenes expressed in the latex of Hevea brasiliensis
260			_bTree Physiology _c2000
300			_a503-510
520			_aRubber (cis-1,4-polyisoprene), an important raw material for many industrial uses, is synthesized in the latex of Hevea brasiliensis (Willd. ex A.Juss) Mull. Arg. We postulated that the genes uniquely or preferentially expressed in the latex may be important for rubber biosynthesis. We constructed cDNA libraries from the latex of H. brasiliensis to investigate the genes expressed in the latex by single-run partial sequencing of the cDNA clones. Sequence analyses identified 245 expressed sequence tags (ESTs), of which 57;showed homology to previously described sequences in public databases. About 16;of the database-matched ESTs encode rubber biosynthesis-related proteins such as rubber elongation factor(REF) and small rubber particle protein(SRPP). The second most frequent transcripts next to rubber biosynthesis-related genes were defense genes and protein metabolism-related genes (12.6;each). About 27;of the database-matched ESTs had sequence homology with genes of unknown function. Among the redundantly expressed genes, REF was the most frequently expresed(6.1;), followed by SRPP(3.7;) and HbLAR(2.9;). Northern blot analyses showed that ten(71;) of the 14 ESTs studied were expressed at a higher level in latex than in leaves.
650			_aBiosynthesis
650			_aEST
650			_aGenetic engineering
650			_aHevea brasiliensis
700			_aChow K S
700			_aKim In Jeong
700			_aOh Soo Kyung
700			_aShin Dong Ho
700			_aYang Jaemo
942			_cJS
999			_c59773 _d59773