000			02002nam a2200265Ia 4500
008			220216s9999 xx 000 0 und d
100			_aRekha K
245		0	_aExpression of stress tolerance in transgenic callus integrated with osmotin gene in Hevea brasiliensis
260			_bRubber Science _c2016
300			_a140-152
520			_aStressful environment is a limiting factor for rubber productivity in the traditional rubber growing areas and limits the expansion of rubber cultivation to newer areas in several rubber producing countries, including India. Conventional methods of Hevea breeding take several years to produce plants with desirable traits. Genetic transformation offers a viable approach for producing plants with desired traits within a short period of time. Since osmotin gene is reported to impart tolerance to abiotic and biotic stress, the present study on Agrobacterium mediated gene transfer was carried out with the gene encoding osmotin protein. Osmotin gene construct with CaMV 35S promoter and kanamycin as selectable marker was used for the purpose. Transformation frequency of 48 per cent was obtained from anther derived embryogenic calli. Molecular analysis with PCR and RT-PCR could confirm the integration of gene and its expression. The calli were subjected to PEG as well as salt stress for fixed intervals. Higher proline accumulation in tansgenic cell lines compared to control was observed under PEG stress. Transgenic cell lines could tolerate higher concentration (50 mM). The regenerated plants from these transgenic lines are expected to perform well under stressful environments.
650			_aGenetic transformation
650			_aHevea brasiliensis
650			_aOsmotin
650			_aProline estimation
650			_aStress tolerance
700			_aAkshara S R
700			_aJayashree R
700			_aMathew S A
700			_aNazeem P A
700			_aSobha S
700			_aSushamakumari S
700			_aVenkatachalam P
942			_cJS
999			_c59178 _d59178