000			02203nam a2200253Ia 4500
008			220216s9999 xx 000 0 und d
100			_aAmbily P.K
245		0	_aExpression analysis of rubber biosynthetic pathway genes in Hevea brasiliensis
260			_bJournal of Plantation Crops _c2018
300			_a102-111
520			_aHevea brasiliensis, the primary commercial source of natural rubber (cis-1, 4-polyisoprene), is a fundamental raw material used for manufacturing a wide range of industrial and domestic rubber products in automobile, medical and defense industries. In Hevea, biosynthesis of rubber takes place through mevalonate pathway. Clonal variations in the productivity of rubber may be the result of variations in the activities of the enzymes involved in rubber biosynthesis in different Hevea clones. In this study, expression of 14 genes corresponding to enzymes/regulatory proteins involved in rubber biosynthesis was analyzed in high and low latex yielding clones of Hevea brasiliensis. The level of expression of HbSUT3, a sucrose transporter and enzymes related to the synthesis of rubber such as hydroxymethyl glutaryl-CoA synthase (hmgs), HMG-CoA reductase (hmgr) and mevalonate diphosphate decarboxylase (MVD) were found to be significantly higher in high rubber yielding clones compared to the low rubber yielding clones. The higher expression of these genes might result in an increased supply of IPP, the isoprenoid monomer, required for rubber biosynthesis. Expression of genes in the downstream rubber biosynthetic pathway such as FPPS, RuT and REF2 were also found to be significantly higher in high rubber yielding clones than low yielders. The results suggest that high rubber yield is associated with high expression of these genes and these genes can be used as markers for high yield potential in Hevea.
650			_aGene expression
650			_aHevea brasiliensis
650			_aHMG-CoA reductase
650			_aHMG-CoA synthase
650			_aRubber biosynthesis
650			_aSucrose transporter
700			_aAnnamalainathan K
700			_aJacob J
700			_aKrishnakumar R
700			_aSreelatha S
700			_aThomas M
942			_cJS
999			_c59169 _d59169