000			01524nam a2200253Ia 4500
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100			_aQiang Li
245		0	_aEfficient editing of an adenoviral vector genome with CRISPR/Cas9
260			_bIndian Journal of Microbiology _c2021
300			_a91-95
500			_aSource Year: 2021
520			_aImmunotherapy based on genetic modification of T cells has played an important role in the treatment of tumors and viral infections. Moreover, adenoviral vectors engineered with improved safety due to their inability to engineered with improved safety due to their inability to integrate into the host genome have been key in the clinical application of T cells therapy. However, the commonly used adenoviral vector Ad5 exhibits low efficiency of infection of human T cells and the details of the intracellular trafficking pathway of adenoviral vectors in human primary T cells remains unclear. Resolution of these issues will depend on successful modification of the adenoviral vector. To this end, here we describe the successful establishment of a simple and efficient method for editing adenoviral vectors in vitro using the CRISPR-Cas9 gene editing system to target the adinoviral fiber gene.
650			_aAdenoviral vector
650			_aCRISPR-Cas9
650			_aT-cell therapy
700			_aChen Z
700			_aChen Z
700			_aGong C-y
700			_aShao Hong-w
700			_aWang H
700			_aYang J-x
700			_aZhang W-f
942			_cJS
999			_c58427 _d58427