000			01291nam a2200193Ia 4500
008			220216s9999 xx 000 0 und d
100			_aYun W Z
245		0	_aEffect of temperature on stamen culture and somatic plant regeneration in rubber
260			_bActa Agronomica Sinica _c1995
300			_a723-726
500			_aSource Year: 1996
520			_aIn stamen culture experiments, a temperature difference of only 2 degree C could cause marked differences in efficiency. High temperatures could enhance callus formation rate but reduce callus differentiation ability, resulting in a lower plantlet induction rate owing to reduced embryoid quantity and quality. They could also accelerate root development, causing the root to appear before the shoot in some cases and thus impairing further embryoid development. Optimum temperatures were 24 to 27 degree C depending on the stage of culture. The temperatures most suitable for callus induction, somatic embryogenesis and plant regeneration were (respectively) 26, 24-25 and 26-27 degree C. Regeneration frequencies as high as 40.5;were obtained by using these optimum temperatures.
650			_aRubber
650			_aSomatic plant regeneration
650			_aStamen culture
700			_aTing C X
856			_uPBA
942			_cJS
999			_c58192 _d58192