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008			220216s9999 xx 000 0 und d
100			_aLardet Ludovic
245		0	_aEffect of exogenous calcium on post-thaw growth recovery and subsequent plant regeneration of cryopreserved embryogenic calli of Hevea brasiliensis (Muell. Arg)
260			_bPlant Cell Reports _c2007
300			_a559-569
520			_aA reliable cryopreservation technique was developed for friable embryogenic callus lines of Hevea brasiliensis. The study showed that reducingthe CaCl2 concentration of the pre-culture medium from 9mM to 1 or 0 mM CaCl2 before cryopreservation promoted post-thaw callus growth, 1 mM being the optimum CaCl2 concentration for embryo regeneration. Post-thaw callus proliferation decreased in line with the increase of planted callus weight. The effect of cryopreservation was assessed on 39 independent lines showing that cryopreservation did not affect embryogenic and plant regeneration for a majority of lines. The decrease in CaCl2 concentration of the pre-culture medium and the endogenous calcium content of the calli. It also highlighted the implication of tissue calcium content in cryotolerance. Callus water status and the different ways by which calcium could prevent cryoinjury is also discussed.
650			_aCalcium
650			_aCryopreservation
650			_aEmbryogenic culture
650			_aPre-culture
700			_aCarron Marc-Philippe
700			_aDessailly Florence
700			_aMartin Florence
700			_aMontoro Pascal
942			_cJS
999			_c57833 _d57833