000			01430nam a2200217Ia 4500
008			220216s9999 xx 000 0 und d
100			_aJayasree R
245		0	_aEffect of colchicine on callus induction from isolated microspores in Hevea brasiliensis
260			_bPlant Cell Biotechnology and Molecular Biology _c2007
300			_a89-92
500			_aSource Year: 2007
520			_aA method for the induction of haploid callus from isolated microspores of Hevea brasiliensis has been developed. The growth promoting effects of colchicine on callus formation was proved. Anthers at the late uni-nucleate to the early bi-nucleate stage of the microspores were pretreated at 4 degree C for 5-7 days before microspore isolation. Different durations of colchicine exposure (24-96 hrs) as well as varying concentrations (100-1000 mg/l) were tested after the pre-culture. The maximum response was noticed when the microspores were pre-cultures in 100 mg/l colchicine for 96 hrs. It was observed that a high sucrose concentration (180g/l) favoures callus growth in the presence of the growth regulators, kinetin and zeatin (3.0 mg/l). This is a major step towards utilization of haploid technology in Hevea breeding.
650			_aAndrogenesis
650			_aColchicine
650			_aIsolated microspores
700			_aJayasree P K
700			_aRekha K
700			_aSanju R
700			_aThulaseedharan A
942			_cJS
999			_c57749 _d57749