000			01205nam a2200169Ia 4500
008			220216s9999 xx 000 0 und d
100			_aMontoro P
245		0	_aDurable somatic embryogenesis in Hevea brasilisensis
260			_bRevue de Cytologie et de Biologie Vegetales. Le Botaniste (France) _c1994
300			_a113-119
500			_aSource Year: 1996
520			_aVarious culture parameters were tested with Hevea brasiliensis to stimulate the friability of callus without altering its embryogenic capacity. An imbalance in the auxin/cytokinin balance in the culture medium promotes acquisition of friability to the detriment of meristematic activity. On the other hand high concentrations of Ca or saccharose prroduce friable callus composed essentially of embryogenic and pro-embryonic cells. Friable callus was obtained with 3 clones of Hevea, PB 260, PR 107 and GT1, on an Ca-enriched medium, making it possible to grow suspended cultures. During several months of maintenance, these cultures can regenerate somatic embryos and plantlets when transplanted on a solid medium of compact cellular aggregates.
700			_aCarron M P
700			_aEtienne H
856			_uAEDR
942			_cJS
999			_c57433 _d57433