000 01478nam a2200181Ia 4500
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100 _aZhi Deng
245 0 _aDifferences of phospholipase A2 gene expression in latex
260 _bProceedings of IRRDB Conference: NR Industry: Responding to Globalization, 7-8 September 2004, Kunming International Convention & Exhibition Center, China. pp.115-118.
520 _aThe phospholipase A2 gene partial cDNA sequence was amplified by RT-PCR in latex. The sequence analysis displayed that it was identical with the cDNA sequence of hev b7 published by Kostyal D.A (1998). Hev b7 was similar to patatin and had phospholipase A2 gene indicated that the expression level of phospholipase A2 gene was different under various treatments. In the young tree experiment, it showed that the intensity of hybridization signal of the primary tapping was the weakest, while the intensity of hybridization signal of the first tapping with Ethrel treatment was stronger than that of the first tapping without Ethrel treatment. In the mature tree experiment, the clone Reyan7-33-97(Ethrel) was the strongest among four treatments, and the clone Reyan7-33-97 (no Ethrel) was stronger than the clone PR107(Ethrel), while the clone PR107(no Ethrel) was the weakest.
650 _aEthrel
650 _aHevea brasiliensis
650 _aPhospholipase A2
650 _aWounding
700 _aRizhong Zeng
700 _aXianzhou Xiao
942 _cJS
999 _c57173
_d57173