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100 _aNayaka Ngobisa A I C
245 0 _aCultural and morphological characterisations of Fusicoccum sp., the casual agent of rubber (Hevea brasiliensis) leaf blight in Malaysia
260 _bJournal of Rubber Research
_c2012
300 _a64-79
500 _aSource Year: 2012
520 _aLeaf blight caused by Fusicoccum sp is becoming a serious canopy disease of rubber trees in Malaysia. Despite its increasing significance, information regarding the biology of the casual fungus is scarce. This study provides morphological data useful for its characterisation. Fungal isolates obtained from leaf samples collected from several rubber plantations in Selangor, Johor and Perak were compared in terms of their behaviour in various cultural media potato dextrose agar (PDA), Czapex-dox agar (CDA), corn meal agar (CMA) and malt extract agar (MEA). Furthermore, their incidence on rubber leaves was assessed. In all media and different pH tested, significant differences were observed in spore production, dimension of conidiomata and growth rates among isolates. On PDA, pH 6 supported the best growth compared to other media used. The growth rate was 16.8 mm/day on PDA and MEA, against 14.4 mm/day on CDA and 11.4 mm/day on CMA. The highest spore production (161.33 x 10 6 spores/mL) was obtained on PDA. The optimum temperature for spore germination in water agar was 24 degree C - 28 degree C within a period of eight hours. All fungal isolates tested were pathogenic to Hevea brasiliensis but showed variation in symptoms.
650 _aDisease incidence
650 _aFusicoccum sp
650 _aLeaf blight
650 _aSporulation
700 _aMurnita M M
700 _aWong M Y
700 _aZainal Abidin M A
942 _cJS
999 _c56608
_d56608