000			01369nam a2200217Ia 4500
008			220216s9999 xx 000 0 und d
100			_aEngelmann F
245		0	_aCryopreservation of embryogenic calluses of two commercial clones of Hevea brasiliensis
260			_bCryo-letters _c1997
300			_a107-116
500			_aSource Year: 1998
520			_aTwo cryopreservation protocols, one using a classical freezing process and the other a simplified freezing process, were developed for embryogenic calluses of a commercial clone of Hevea, PR107. After preculture with 1 M sucrose and 10;dimethyl sulfoxide, embryogenic calluses were frozen in a programmable freezer at 0.2 degree C min-1 down to -40 degree C, or in a simple device consisting of an isopropanol bath enclosed in a polystyrene box, placed in a -80 degree C deep-freezer, thus achieving an average cooling rate of 0.2 degree C min-1 down to -40 degree C. High survival and rapid regrowth, as well as production of somatic embryos, were obtained with calluses cryopreserved using both freezing protocols. The simple freezing protocols was successfully used with a second commercial clone.
650			_aCryopreservation
650			_aHevea brasiliensis
700			_aCarron M P
700			_aChabrillange N
700			_aEtienne H
700			_aLartaud M
856			_uIRRDB
942			_cJS
999			_c56587 _d56587