000 00904nam a2200205Ia 4500
008 220216s9999 xx 000 0 und d
100 _aSafiah Atan
245 0 _aConstruction of a microsatellite-enriched library from Hevea brasiliensis
260 _bJournal of Natural Rubber Research
_c1996
300 _a247-255
520 _aA Microsatellite library was constructed from Hevea brasiliensis by cloning DNA fragments of between 200 bp-800 bp in length. These were ligated to pBluescript KS + phagemid as the vector and transformed into Escherichia coli DH5 alpha. The library appeared to be highly enriched with simple sequence repeats; (GACA)n - 10;, (GATA)n - 9;, (GA)n - 34;and (GC)n - 9;.
650 _aDNA extraction
650 _aHevea brasiliensis
650 _aMicrosatellite library
650 _aPCR
650 _aRFLP
700 _aLow F C
700 _aSaleh N M
942 _cJS
999 _c56209
_d56209