000			01495nam a2200205Ia 4500
008			220216s9999 xx 000 0 und d
100			_aPeng Shi-Qing
245		0	_aCloning and characterization of a novel cysteine protease gene (HbCP1) from Hevea brasiliensis
260			_bJournal of Bioscience _c2008
300			_a681-690
520			_aThe full-length cDNA encoding a cysteine protease, designated HbCP1, was isolated for the first time from Hevea brasiliensis by the rapid amplification of cDNA ends (RACE) method HbCP1 contained a 1371 bp open reading frame encoding 457 amino acids. The deduced HbCP1 protein, which showed high identity to cysteine proteases of other plant species, was predicted to pocesses a putative repeat in toxin (RTX) domain at the N-terminal and a granulin (GRAN) domain at the C-terminal. Southern blot analysis indicated that the HbCP1 gene is present as a single copy in the rubber tree. Transcription pattern analysis revealed that HbCP1 had high transcription in laticifer, and low transcription in bark and leaf. The transcription of HbCP1 in latex was induced by ethylene and tapping. Cloning of the HbCP1 gene will enable us to further understand the molecular characterization of cycteine protease and its possible function in the rubber tree.
650			_aCysteine protease
650			_aGene expression
650			_aLatex
650			_aStress
700			_aLi Hui-Liang
700			_aTian Wei-Min
700			_aZhu Jia-Hong
942			_cJS
999			_c55769 _d55769