000			01547nam a2200181Ia 4500
008			220216s9999 xx 000 0 und d
100			_aJayasinghe C K
245		0	_aCell wall degrading enzyme secretion by cylindrocladium quinqueseptatum
260			_bJournal of the Rubber Research Iinstitute of Sri Lanka _c1999
300			_a47-60
520			_aCylindrocladium quinqueseptatum causes leaf fall of clove in Sri Lanka and is responsible for the leaf spots of rubber in Malaysia. Only one isolate (Kp) from four pathogenic isolates examined secreted polygalacturanase (PG) when grown in liquid media. None of the isolates secreted pectin lyase (PL). The extracts of clove and rubber leaf tissues, inoculated with the Kp isolate did not show any PG and PL activity. The leaf tissue did not have the ability to inhibit C.quinqueseptatum PG produced in culture. All isolates of C.quinqueseptatum secreted cellulases viz. cellobiase and B-glucosidase in culture. Cellobiase was detected in the inoculated rubber leaves. The leaves of B-glucosidase, an inherent enzyme of Hevea leaves, also increased rapidly following infection. The results indicate that pectic enzymes play a minimal or no role in disease development of C.quinqueseptatum infection in cloves and Hevea. Thus cellulolytic enzyme probably play a nutritive role.
650			_aCelluloitic enzymes
650			_aCylindrocladium quinqueseptatum Eugenia caryophyllata
650			_aHevea brasiliensis
650			_aPectolytic enzymes
700			_aWijesundera R L C
942			_cJS
999			_c55253 _d55253