000			01708nam a2200229Ia 4500
008			220216s9999 xx 000 0 und d
100			_aSunderasan E
245		0	_aCell viability assay guided fractionation of natural rubber latex sera
260			_bJournal of Rubber Research _c2013
300			_a195-202
500			_aSource Year: 2013
520			_aNatural rubber latex sera have been investigated for anti-proliferative property on cancer cell lines. The present report describes fractionaation of latex B- and C-serum using cell viability assay(anti-proliferative activity) as a guide to narrow down on the active constituents. Dialysis of B-and C-serum against distilled water caused some of its constituents to precipitate, then a brief centrifugation separated their fractions- dialysed B-serum percipitate (DCP) nad dialyesd C-serum supernanatant (DCS). Boiling of B- and C-serum resulted in extensive precipitation; a brief centrifugation separated the boiled B-and C-serum precipitate from their supernantant. Cell viability assy performed on two human cancer-origin cell lines revealed a marked increase in anti-proliferative activity in the dialysed C-serum fractions and in DBP but diminished in DBS as well as in the boiled B-and C-serum fractions. Chromatographic separation of DBP, DCP and DCS followed by cell viability assy will shed light if sub-fractions with further improved anti-proliferative activity are attainable.
650			_aanti-proliferative activity
650			_aCell viability assay
650			_aLatex B-serum
650			_aLatex C-serum
700			_aLam K L
700			_aOng M T
700			_aRahman Norazreen Abd
700			_aYang K L
942			_cJS
999			_c55251 _d55251