000			02052nam a2200217Ia 4500
008			220216s9999 xx 000 0 und d
100			_aAbraham T
245		0	_aAssociation of a viroid with tapping panel dryness of Hevea brasiliensis and its molecular characterization
260			_bSustainability of the NR Industry: Issues, Challenges & Opportunities: International Rubber Conference, 21-22 November 2016, Cambodian Rubber Research Institute (CRRI) and International Rubber Research and Development Board (IRRDB), Siem Reap, Cambodia, pp.396-408.
520			_aTapping panel dryness (TPD) syndrome is a serious malady of rubber (Hevea brasiliensis) with unknown etiology occurring in all rubber growing areas resulting in partial to complete drying of tapping panel. The possibility of association of viroid with TPD syndrome was investigated in the present study. An infectious LMW RNA was detected from different samples drawn from varying ages of trees from different locations. The consistency in the presence of LMW RNA over three years of observation in TPD affected trees and its absence in majority of the healthy ones points to the association of the LMW RNA with TPD. About 30 per cent of the apparently healthy trees which may be symptomless carriers also showed presence of LMW RNA. Such trees later showed TPD symptoms. Presence of bands in apparently healthy samples indicates that the biotic agent can be detected in the tree much before the TPD symptoms are visible. The PCR of the cDNA using viroid specific primers consistently amplified products in the range of viroids in R-PAGE positive TPD affected samples but was absent in R-PAGE negative apparently healthy samples. The amplified LMW RNA showed 98;sequence homology to Potato Spindle Turber Viroid (PSTVd) on BLAST analysis.
650			_aLMW RNA
650			_aSymptomless carriers
650			_aTPD
650			_aViroid
700			_aJacob C K
700			_aKumar A
700			_aMalathi V G
700			_aMathew J
700			_aRamachandran P
942			_cJS
999			_c54568 _d54568