000			01389nam a2200241Ia 4500
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100			_aSathik M B M
245		3	_aAn Efficient method for the isolation of good quality RNA from bark tissues of mature rubber trees
260			_bJournal of Rubber Research _c2005
300			_a182-189
520			_aIn this study, an attempt has been made to extract good quality RNA from soft bark tissues of Hevea which generally contain plenty of interfering compounds such as phenolic compounds, tannins, lignins and carbohydrates. The method explained here involes removal of phenolic compounds using polyvinyl pyrrolidone and selective precipitation of RNA using lithium chloride. The purity of RNA prepared by this method was tested for its ability to get a good quality cDNA prepared. The cDNA produced from total RNA preparation was further con firmed by amplification of specific house keeping genes using quantitative real time PCR method. This protocol appears best suited for Hevea bark that is rich in several contaminating factors.
650			_acDNA
650			_aReal time PCR
650			_aRecalcitrant bark tissue
650			_aRRII 105
650			_aTapping panel dryness
650			_aTotal RNA isolation
700			_aJacob J
700			_aNataraja K N
700			_aThomas M
700			_aUdayakumar M
942			_cJS
999			_c54121 _d54121