000			01407nam a2200241Ia 4500
008			220216s9999 xx 000 0 und d
100			_aLicy J
245		3	_aAn Approach to identify disease resistance gene analogues in Hevea
260			_bIndian Journal of Natural Rubber Research _c2000
300			_a79-85
520			_aA preliminary attempt was made to identify possible disease resistance gene analogues in rubber. A procedure was developed for the extraction of high quality DNA from rubber leaves, which can be used for the polymerase chain reaction (PCR) amplification of DNA. Eighteen primers, designed based on homologies between known resistance genes, were used in various combinations to amplify sequences from rubber cultivar FX 516, which is resistant to Phytophthora leaf fall disease and cultivar RRII 105, which is tolerant. The PCR products were cloned into plasmid vectors and the cloned inserts were sequenced. Although none of the clones obtained had high homology to resistance gene sequences, the putative protein encoded by one sequence had some homology to hem N gene.
650			_aCloning
650			_aHevea
650			_aPhytophtora leaf fall
650			_aPOlymerase chain reaction
650			_aResistance genes
700			_aBligh F J
700			_aDavey M R
700			_aDickinson J
700			_aPower J B
700			_aSaraswathyamma C K
942			_cJS
999			_c54100 _d54100