000			02113nam a2200193Ia 4500
008			220216s9999 xx 000 0 und d
100			_aYip Esah
245		0	_aAllergenicity of latex gloves with reference to latex protein sensitive individuals in a canadian population
260			_bJournal of Rubber Research _c2000
300			_a129-141
520			_aAllergenicity of latex gloves with varying levels of total extractable proteins was studied, by evaluating their allergic responses elicited, if any, in latex sensitive individuals of Canadian origin. Total extractable protein (EP) content of 30 latex medical gloves (powdered and powder-free) was determined by the modified Lowry method, according to both the Rubber Research Institute of Malaysia (RRIM) and the American Society for Testing and Materials (ASTM) protocols. While EPrrim values ranged from 865mg/g to <10mg/g, EPastm values varied from 870mg/g to <30 mg/g. A total of 30 latex allergic volunteers were skin-prick tested with the glove extracts. Results showed that gloves with high extractable protein contents always tend to elicit positive allergic reactions. On the other hand, much reduced or no allergic responses were associated with gloves having low extractable proteins, regardless of whether the gloves were powdered or powder-free. More than 60;of negative allergic responses were consistently observed at EPrrim of about 100mg/g and lower, and at EPastm of less than 50mg/g. The total extractable protein content of latex gloves was found to be significantly correlated to their allergenicity, notwithstanding that the protein measurements were not fully allergen specific. Correlation coefficients r of 0.94 for EPrrim and 0.88 for EPastm, were obtained. This finding confirms an earlier report that showed close correlation between allergenicity and EPrrim in a study based on a Finnish population of different genetic make-up.
650			_aAllergenicity
650			_aExtractable protein
650			_aLatex gloves
650			_aLowry method
650			_aMedical gloves
700			_aSussman Gordon L
942			_cJS
999			_c54046 _d54046