000			02179nam a2200217Ia 4500
008			220216s9999 xx 000 0 und d
100			_aJayasree P K
245		0	_aAgrobacterium-mediated transformation of Hevea brasiliensis with apple cDNA encoding sorbitol-6-phosphate dehydrogenase
260			_bRubber Science _c2015
300			_a31-39
520			_aThe increasing demand of natural rubber abd land unavailability in traditional regions has been resulted the cultivation of rubber in non-traditional regions. However, the severe agro-climatic conditions in non-traditional regions adversely affected growth and yield of rubber. In this context, scope for the introduction of genes conferring tolerance to abiotic stresses is of paramount importance and hence the study was undertaken. Embryogenic callus derived from immature anther of Hevea brasiliensis, clone RRII 105 was used as the target tissue for genetic transformation. Kanamycin sensitivity to embryogenic callus was examined by kill curve test and medium with 100 mgL-1 kanamycin completely suppresses the growth of untransformed callus and was selected as the suitable concentration for selection of transformants. Addition of ABA was found to be beneficial for embryogenesis and maximum embryo induction was obtained with 2.0 mgL-1. Among various levels of sucrose (2-10;), 4 per cent sucrose enhanced embryo induction efficiency and gradually decreased by higher levels. Mature embryos get germinated and converted into plantlets. GUS histochemical assay revealed expression of uidA gene in transformed callus and embryos which was evidenced by the intense blue colour. Polymerase chain reaction confirmed the presence of S6PDH, uidA and nptII genes in transformed callus and embryos. The transgenes were also detected in plasmid DNA (positive control), but absent in untransformed callus (negative control).
650			_aAgrobacterium tumefaciens
650			_aGenetic transformation
650			_aGUS histochemical assay
650			_aPCR analysis
650			_aSorbitol-6-phosphate dehydrogenase gene
700			_aDivya S
700			_aSupriya R
700			_aThulaseedharan A
942			_cJS
999			_c53977 _d53977