000			01489nam a2200205Ia 4500
008			220216s9999 xx 000 0 und d
100			_aArokiaraj P
245		0	_aAgrobacterium-mediated transformation of Hevea anther calli and their regeneration into plantlets
260			_bJournal of Natural Rubber Research _c1996
300			_a77-87
520			_aA gene transfer system for Hevea brasiliensis was estabilished with Agrobacterium tumefaciens GV 2260 (p35SGUSINT) and LBA4404 (pAL4404/pMON9793). In this system Hevea brasiliensis anther-derived calli were transformed with vectors harbouring the B-glucuronidase (gus) gene and the neomycin phosphotransferase (nptII) gene. The success of gene transfer was determined by histochemical staining and fluorometric assay for B-glucuronidase activity and enzyme linked immunosorbent assay for detecting neomycin phosphotransferase II protein levels. These indepedent assays all showed a several-fold increase, compared to control values, in enzyme activity and protein levels in extracts from transformed calli and embryoids of Hevea brasiliensis. The presence of the gus gene in transformed plantlets was confirmed by using the polymerase chain reaction protocol as performed on DNA isolated from transformed calli and embryoids.
650			_aAgrobacterium
650			_aAnther calli
650			_aHevea brasiliensis
700			_aCharlwood B V
700			_aCoomber S
700			_aJaafar H
700			_aJones H
942			_cJS
999			_c53976 _d53976