000			01612nam a2200181Ia 4500
008			220216s9999 xx 000 0 und d
100			_aSuvachittanont W
245		0	_a3-Hydroxy-3-methylglutaryl-coenzyme, a synthase in Hevea brasiliensis
260			_bPhytochemistry _c1995
300			_a757-761
500			_aSource Year: 1996
520			_a3-Hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) synthase activity in rubber (H. brasiliensis) latex (collected from 15-year-old trees of clone RRIM 600 growing at the Songkal Rubber Research Centre) and in fractions of latex obtained by centrifugation was deterrmined using a radiochemical method. The enzyme was found in both C-serum and bottom fraction, but most was in the C-serum. H. brasiliensis leaves showed very low enzyme activity. HMG-CoA synthase in C-serum was free of HMG-CoA lyase. The enzyme in C-serum was stable at -70 C. The formation of HMG-CoA did not depend on a supply of exogenous acetoacetyle-CoA, and the rate of formation was increased as the concentration of acetyl-CoA was increased. The Km for acetyl-CoA was 9mM. The enzyme in C-serum was inhibited by several divalent cations, p-chloromercuribenzoate and dithiothreitol. Diurnal variation in the activity of the enzyme in both C-serum and extracts of leaves was observed. A positive correlation between the enzyme activity and the rubber content of the latex suggested that the enzyme regulates the synthesis of rubber in the latex.
650			_a3-hydroxy-3-methylglutaryl-coenzyme
650			_aHevea brasiliensis
700			_aWititsuwannakul R
856			_uHOA
942			_cJS
999			_c53247 _d53247