In the production process of natural rubber(NR), large quantities of wastewater containing substantial amounts of L-quebrachitol are discharged. To date, published methods of extracting L-quebrachitol from wastewater are still at the laboratory stage. In this study membrane separation technology was applied to the extraction of L-querbrachitol from wastewater and the experimental parameters were studied in order to determine optimal experimental conditions. Results show that the optimum source material for L-querbrachitol extraction was the serum collected by sequeezing the latex coagulum. When this serum was passed through a microfilteration (MF) membrane and two ultrafilteration (UF) membranes, majority of the organic impurities were removed. The resulting ultrafiltrate was concentrated by a nanofilteration(NF) membrane and washed twice. Finally, a new and convenient approch for large scale extraction of L-quebrachitol is proposed combining membrane separation and crystallisation. The output achieved with this approach would be 10 Kg per year under laboratory conditions with 99.20;purity. The structure of L-quebrachitol was confirmed by elementary analysis, infrared (IR) spectroscopy, nuclear magnetic resonance (NMR) spectroscopy, mass spectroscopy (MS) and single-crystal X-ray diffraction.