Hevea brasiliensis (Para rubber tree) is a commercially cultivated crop for latex production worldwide. Rubber trees from
Southern states of India have been afected by Circular Leaf Spot (CLS) disease caused by Colletotrichum fructicola and
Colletotrichum siamense causing yellowing and severe defoliation in rubber plantations in recent years. In this study, we
collected infected leaf samples of CLS disease from diferent locations in the South Indian states of Kerala, Tamil Nadu,
and Karnataka. Colletotrichum fructicola and C. siamense were identifed as the causative agents of CLS disease and the
isolate, C. fructicola was used for genomics analysis. We performed whole-genome sequencing (WGS) of, C. fructicola
and carried out gene content analysis by comparing it with other reported Colletotrichum species. In silico analysis
revealed a rich repertoire of pathogenicity genes and CAZymes in C. fructicola encoding secreted proteins, efectors, plant
cell wall degrading enzymes, and secondary metabolism-associated proteins with possible roles in the host-specifc
interaction and pathogenicity. Our dataset provides a rich resource for understanding the pathogenicity-related genes
that are involved in CLS disease progression and gives a basis for subsequent functional genomics research in
C. fructicola.