Approaches to identify biotic stress resistance gene analogues in Hevea
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TextPublication details: Proceedings of IRRDB Symposium 1999, Hainan, Peoples Republic of China. pp.353-363.Subject(s): Summary: A preliminary attempt has been made to identify possible biotic stress tolerance gene analogues in rubber. An alternate methodology for DNA extraction from rubber leaves was standardised. 18 resistance gene analogues (RGA) were used as primers with a view to identifying biotic stress tolerance in rubber. Two clones, RRII 105 and FX 516 which are tolerant to Phytophthora leaf fall disease were selected for the study. The RGA-PCR strategy points towards the applicability of identifying and sequencing valuable resistance genes which would be of help in designing suitable primers for future investigations. Identification and cloning of resistant genes could find application in evolving transgenic plants.
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Journals
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RRII Library Biotechnology | Journals |
A preliminary attempt has been made to identify possible biotic stress tolerance gene analogues in rubber. An alternate methodology for DNA extraction from rubber leaves was standardised. 18 resistance gene analogues (RGA) were used as primers with a view to identifying biotic stress tolerance in rubber. Two clones, RRII 105 and FX 516 which are tolerant to Phytophthora leaf fall disease were selected for the study. The RGA-PCR strategy points towards the applicability of identifying and sequencing valuable resistance genes which would be of help in designing suitable primers for future investigations. Identification and cloning of resistant genes could find application in evolving transgenic plants.
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