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Cultural and morphological characterisations of Fusicoccum sp., the casual agent of rubber (Hevea brasiliensis) leaf blight in Malaysia

By: Contributor(s): Material type: TextTextPublication details: Journal of Rubber Research 2012Description: 64-79Subject(s): Summary: Leaf blight caused by Fusicoccum sp is becoming a serious canopy disease of rubber trees in Malaysia. Despite its increasing significance, information regarding the biology of the casual fungus is scarce. This study provides morphological data useful for its characterisation. Fungal isolates obtained from leaf samples collected from several rubber plantations in Selangor, Johor and Perak were compared in terms of their behaviour in various cultural media potato dextrose agar (PDA), Czapex-dox agar (CDA), corn meal agar (CMA) and malt extract agar (MEA). Furthermore, their incidence on rubber leaves was assessed. In all media and different pH tested, significant differences were observed in spore production, dimension of conidiomata and growth rates among isolates. On PDA, pH 6 supported the best growth compared to other media used. The growth rate was 16.8 mm/day on PDA and MEA, against 14.4 mm/day on CDA and 11.4 mm/day on CMA. The highest spore production (161.33 x 10 6 spores/mL) was obtained on PDA. The optimum temperature for spore germination in water agar was 24 degree C - 28 degree C within a period of eight hours. All fungal isolates tested were pathogenic to Hevea brasiliensis but showed variation in symptoms.
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Item type Current library Vol info Status
Journals Journals RRII Library Pathology Volume 15, Issue 1 Journals
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Source Year: 2012

Leaf blight caused by Fusicoccum sp is becoming a serious canopy disease of rubber trees in Malaysia. Despite its increasing significance, information regarding the biology of the casual fungus is scarce. This study provides morphological data useful for its characterisation. Fungal isolates obtained from leaf samples collected from several rubber plantations in Selangor, Johor and Perak were compared in terms of their behaviour in various cultural media potato dextrose agar (PDA), Czapex-dox agar (CDA), corn meal agar (CMA) and malt extract agar (MEA). Furthermore, their incidence on rubber leaves was assessed. In all media and different pH tested, significant differences were observed in spore production, dimension of conidiomata and growth rates among isolates. On PDA, pH 6 supported the best growth compared to other media used. The growth rate was 16.8 mm/day on PDA and MEA, against 14.4 mm/day on CDA and 11.4 mm/day on CMA. The highest spore production (161.33 x 10 6 spores/mL) was obtained on PDA. The optimum temperature for spore germination in water agar was 24 degree C - 28 degree C within a period of eight hours. All fungal isolates tested were pathogenic to Hevea brasiliensis but showed variation in symptoms.

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