Development of RAPD markers in tritordeum and addition lines of Hordeum chilense in Triticum aestivum
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TextPublication details: Plant Breeding 1996Description: 52-56Subject(s): Summary: RAPD markers were developed for octoploid XTritordeum (amphiploid Hordeum chilense x Triticum aestivum) and its parents. Two bread wheats, two H.chilense accessions and the two tritordeums synthesized with them were used. A total of 41 arbitrary decamer primers were tested, yielding 190 products that could be assigned to wheat, 185 to H. chilense and 108 that were nonspecific (present in wheat and barley). A total of 44 products were specific to one H. chilense line and 33 to the other 16 of the former were located on the chromosomes using a set of H. chilense in T. aestivum addition lines. The potential of RAPDs for developing addition lines or the detection of introgressions of H. chilense in bread wheat is discussed.
| Item type | Current library | Vol info | Status | |
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Journals
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RRII Library | Volume 115, Issue 1 | Journals |
RAPD markers were developed for octoploid XTritordeum (amphiploid Hordeum chilense x Triticum aestivum) and its parents. Two bread wheats, two H.chilense accessions and the two tritordeums synthesized with them were used. A total of 41 arbitrary decamer primers were tested, yielding 190 products that could be assigned to wheat, 185 to H. chilense and 108 that were nonspecific (present in wheat and barley). A total of 44 products were specific to one H. chilense line and 33 to the other 16 of the former were located on the chromosomes using a set of H. chilense in T. aestivum addition lines. The potential of RAPDs for developing addition lines or the detection of introgressions of H. chilense in bread wheat is discussed.
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